Third-Party Chromogen Compatibility on the Leica BOND RX/RXm

Although we recommend users take advantage of first-party chromogens available from Leica Biosystems, there may be workflows that benefit from the use of third-party chromogens. Our Product Development team continuously evaluates and validates third-party chromogens for use with HCR™ Pro RNA-ISH on the Leica BOND RX/RXm. Below you can find our team's latest recommendations: 

Validated third-party HRP-driven chromogens (last updated: 11/13/23): 

Biocare's DAB Chromogen

• Mixing Ratio: 1:20
• Deposition Time: 5-8 minutes

Biocare's Vina Green Chromogen

• Mixing Ratio: 1:20
• Deposition Time: 5-10 minutes

Abcam's DAB Substrate

• Mixing Ratio: 1:50
• Deposition Time: 5-10 minutes

Enzo's HIGHDEF Yellow IHC Chromogen

• Mixing Ratio: 1:50
• Deposition Time: 10-15 minutes

Validated third-party AP-driven chromogens (last updated: 11/13/23): 

Enzo's HIGHDEF Red IHC Chromogen

• Mixing Ratio: 1:50
• Deposition Time: 10-15 minutes

Biocare's Vulcan Red Chromogen

• Mixing Ratio: 1:50
• Deposition Time: 10-14 minutes

Biocare's Warp Red Chromogen

• Mixing Ratio: 1:50
• Deposition Time: 5-7 minutes